Plant-Lore with Reference to Muslim Folksong in Association with Human Perception of Plants in Agricultural and Horticultural Practices
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CRYOPRESERVATION OF STRAWBERRY SHOOT TIPS BY ENCAPSULATION-DEHYDRATION
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| Source: Not. Bot. Hort. Agrobot. Cluj, XXXIV/2006 |
| Publisher: AcademicPres |
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| Institute of Biological Research, 400015 Cluj-Napoca, 48 Republicii St., Romania; e-mail: halmagyi.a@gmx.net |
Abstract:
In vitro grown strawberry (Fragaria x ananassa Duch.) shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Encapsulated shoot tips were precultured on MS medium supplemented with different sucrose concentrations (0.25, 0.5, 0.75 and 1.0 M) for 24 h and dehydrated (1 to 5 hours) in a sterile laminar air flow prior to direct immersion in liquid nitrogen (-196°C). A maximum of 63% regrowth of cryopreserved shoot tips was obtained following preculture in 0.75 M sucrose solution and 4 h dehydration. Plants produced from control and cryopreserved shoot tips were phenotypically similar.
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